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epigallocathecin gallate  (Rooibos Ltd)

 
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    Rooibos Ltd epigallocathecin gallate
    Epigallocathecin Gallate, supplied by Rooibos Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epigallocathecin gallate/product/Rooibos Ltd
    Average 90 stars, based on 1 article reviews
    epigallocathecin gallate - by Bioz Stars, 2026-05
    90/100 stars

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    epigallocathecin gallate  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology epigallocathecin gallate
    Fig. 5. Inhibition of STAT-1 does not prevent cytokine-induced EndoC-bH1 cell death. (A) EnodC-bH1 cells were treated with control or STAT-1 siRNA. Two days later cells were exposed to IL-1b þ IFN-g overnight and cell death rates were analyzed by flow cytometry. For immunoblot analysis of P-STAT-1 cells were exposed to cytokines for 30 min. Upper panel shows the means ± SEM for 4 experiments and the lower panel shows a 55% knockdown of Phospho-STAT-1 induced by siRNA treatment. The intensities of the P-STAT-1 bands were normalized to total protein loading and transfer, as assessed by amidoblack staining. (B) and (C) EndoC-bH1 cells were pre-exposed to 10 mM <t>epigallocathecin</t> gallate (EGCG) or 50 ng/ml fludarabine (Flud) for 15 min and then cultured for another 18 h in the presence of IL-1b þ IFN-g before analysis of cell death using propidium iodide staining and flow cytometry. Results are means ± SEM for 4 independent observations.
    Epigallocathecin Gallate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epigallocathecin gallate/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    epigallocathecin gallate - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier
    epigallocathecin gallate  (Rooibos Ltd)
    90
    Rooibos Ltd epigallocathecin gallate
    Fig. 5. Inhibition of STAT-1 does not prevent cytokine-induced EndoC-bH1 cell death. (A) EnodC-bH1 cells were treated with control or STAT-1 siRNA. Two days later cells were exposed to IL-1b þ IFN-g overnight and cell death rates were analyzed by flow cytometry. For immunoblot analysis of P-STAT-1 cells were exposed to cytokines for 30 min. Upper panel shows the means ± SEM for 4 experiments and the lower panel shows a 55% knockdown of Phospho-STAT-1 induced by siRNA treatment. The intensities of the P-STAT-1 bands were normalized to total protein loading and transfer, as assessed by amidoblack staining. (B) and (C) EndoC-bH1 cells were pre-exposed to 10 mM <t>epigallocathecin</t> gallate (EGCG) or 50 ng/ml fludarabine (Flud) for 15 min and then cultured for another 18 h in the presence of IL-1b þ IFN-g before analysis of cell death using propidium iodide staining and flow cytometry. Results are means ± SEM for 4 independent observations.
    Epigallocathecin Gallate, supplied by Rooibos Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epigallocathecin gallate/product/Rooibos Ltd
    Average 90 stars, based on 1 article reviews
    epigallocathecin gallate - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

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    Fig. 5. Inhibition of STAT-1 does not prevent cytokine-induced EndoC-bH1 cell death. (A) EnodC-bH1 cells were treated with control or STAT-1 siRNA. Two days later cells were exposed to IL-1b þ IFN-g overnight and cell death rates were analyzed by flow cytometry. For immunoblot analysis of P-STAT-1 cells were exposed to cytokines for 30 min. Upper panel shows the means ± SEM for 4 experiments and the lower panel shows a 55% knockdown of Phospho-STAT-1 induced by siRNA treatment. The intensities of the P-STAT-1 bands were normalized to total protein loading and transfer, as assessed by amidoblack staining. (B) and (C) EndoC-bH1 cells were pre-exposed to 10 mM epigallocathecin gallate (EGCG) or 50 ng/ml fludarabine (Flud) for 15 min and then cultured for another 18 h in the presence of IL-1b þ IFN-g before analysis of cell death using propidium iodide staining and flow cytometry. Results are means ± SEM for 4 independent observations.

    Journal: Molecular and cellular endocrinology

    Article Title: Role of the AMP kinase in cytokine-induced human EndoC-βH1 cell death.

    doi: 10.1016/j.mce.2015.07.015

    Figure Lengend Snippet: Fig. 5. Inhibition of STAT-1 does not prevent cytokine-induced EndoC-bH1 cell death. (A) EnodC-bH1 cells were treated with control or STAT-1 siRNA. Two days later cells were exposed to IL-1b þ IFN-g overnight and cell death rates were analyzed by flow cytometry. For immunoblot analysis of P-STAT-1 cells were exposed to cytokines for 30 min. Upper panel shows the means ± SEM for 4 experiments and the lower panel shows a 55% knockdown of Phospho-STAT-1 induced by siRNA treatment. The intensities of the P-STAT-1 bands were normalized to total protein loading and transfer, as assessed by amidoblack staining. (B) and (C) EndoC-bH1 cells were pre-exposed to 10 mM epigallocathecin gallate (EGCG) or 50 ng/ml fludarabine (Flud) for 15 min and then cultured for another 18 h in the presence of IL-1b þ IFN-g before analysis of cell death using propidium iodide staining and flow cytometry. Results are means ± SEM for 4 independent observations.

    Article Snippet: IKK inhibitor X and epigallocathecin gallate was from Santa Cruz.

    Techniques: Inhibition, Control, Cytometry, Western Blot, Knockdown, Staining, Cell Culture